Foreign ministry spokesman Bahram Ghasemi made the announcement in an August 22 news briefing.
'Russia has no base in Iran and is not stationed here. They conducted this operation and it is finished for now,' Ghasemi was quoted as saying by the semiofficial Tasnim news agency, RFE/RL reports.
Ghasemi added that Russia's use of the base was 'temporary, based on a Russian request.'
He reiterated that Russia 'has no base in Iran.'
Earlier, Iran's defense minister criticized Russia for publicizing its use of the Iranian base for attacks in Syria, saying it was 'kind of show off and ungentlemanly.'
Iran state TV quoted General Hossein Dehghan on August 22 as saying that the 'Russians seek to show that they are a superpower.'
Dehghan's comments come amid a pushback from Tehran after Russia last week started using an Iranian base to launch air strikes in Syria.
Iranian officials have said that Russian planes were refueling at the Shahid Nojeh air base near Hamadan. They said the move was part of a 'strategic cooperation' with Russia.
Tehran and Moscow are supporters of Syrian President Bashar al-Assad, whom they have helped remain in power.
As reported earlier by 1492news.com, Russian long-range aviation bombed in Syria, taking off from an air base in Iran.